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Digestion was performed using 2–3 mL of collagenase media [prepared with 4 mL Dispase (Corning, 354235), 40 μL of 0.05 mg/mL DNase I (Sigma, DN25), 4 μL anti-mouse CD16/32 antibody (Biolegend, 101302), 200 μL collagenase I (Gibco, 17100017), and 10 μM Y-27632 (APExBIO, A3008)]
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In the context of cancer therapy, our results suggest that stimulation of nucleotide synthesis in combination with GSH depletion may be especially toxic
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