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Subsequently, 0.4 mL of a PI staining/lysis solution (0.5% NP-40, 0.5 mM ethylenediaminetetraacetic acid [EDTA]) and 50 μL of RNase A (10 mg/mL in Tris–EDTA buffer, pH 8.0) solution were added
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Histomorphometric analysis of H&E sections showed a decrease in osteoblast number per bone perimeter (N.Ob/B.Pm) in male and female Rras2 −/− mice (Fig
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