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In a cultured cell model, AHCY pharmacological inhibition led to reduced cap methylation and synthesis of polyadenylated RNA, implicating AHCY in both cap methylation of pre-mRNA and transcriptional elongation processes (62)

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A: The recommended sequence includes: Investigate possible test artifacts or structure-based concerns Conduct in vitro mammalian cell gene mutation assay (Mouse Lymphoma Assay (MLA) or HPRT) If in vitro is negative, proceed to in vivo gene mutation assay (Pig-A assay or transgenic rodent mutation assays) Strategic Considerations for Ames-Positive Results Presented at SOT 2025 The presentation addressed a persistent challenge in pharmaceutical development: addressing Ames-positive results for active pharmaceutical ingredients (APIs) or impurities