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After image capture, 2-propanol (3.5 mL) was added to each dish for 10 min to dissolve the oil red O stain and absorbance was quantified in a plate reader set at 492 nm wavelength
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Likewise, there is also the inhibition of the mRNA of inflammatory mediators (iNOS, IL-6, IFN, and TNF-alpha) and the increased expression of HSP 70 and 90 and antioxidant proteins, such as HO-1, NQO-1, glutathione reductase, glutathione peroxidase 2, and GST-pi [4]
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