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NAD+ (Nicotinamide Adenine Dinucleotide)

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Glutathione peroxidase [GPX, EC.1.11.1.9] Glutathione reductase (0.24 units), and 10 mM glutathione (GSH) were added to hemolysate and preincubated for 10 min at 37˚C, followed by the addition of nicotinamide adenine dinucleotide phosphate (NADPH) in 0.1% sodium bicarbonate