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Volume:10mL
To produce these density solutions from OptiPrep™ and to mimic the EDTA profile of the NycodenzⓇ gradient, first dilute OptiPrep™ with an equal volume of PBS to produce a 30% (w/v) iodixanol solution and then dilute further with PBS containing 4 mM EDTA to make solutions of 12%, 14.5%, 17.5% and 20% (w/v) iodixanol
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