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In vitro experiments demonstrated that NNMT overexpression significantly increased Sirt1 protein expression in primary hepatocytes, while NNMT knockdown led to a notable decrease in Sirt1 protein expression

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As a result, we have found a decrease in O 2 • − and H 2 O 2 production as well as lipid peroxidation markers (DC and MDA) in heart mitochondria of old glutathione-treated rats (Table 2)